This study was performed to define the cytotoxicity and the anti-inflammatory action of Pulsatilla koreana ext acts
To analyze cvtotoxic effects, gingival and periodontal ligament fibroblasts were used, and anti-inflanmnmatory actions related to reduction of IL-1β(3 and PGE2 production were performed in vitro. for the suggestion of efficacy and safety on periodontal therapeutic use of Pulsatilla koreana extracts.
We extracted ethylacetate and butylalcohol from well-dried and ground Pulsatilla koreana throughout multiple processing. then used different concentration solution(0.1 %, 0.2 %, 0.4 %, 0.01 %, 0.02 %. 0.04 %, 1 %, 2 %) of ethylacetate and hutylalcohol extracts to examine cytotoxic effects and anti-inflammatory actions
Cytotoxic effects were examined by EUSA reader using MTT(Methyl Thiazol-2-YL-2, 5-Biphenyl Tetrazolium hromide)solution following culture of human gingival and periodontal ligament fibroblasts. Synthesis of IL-1β(3 was examined by IL-1β enzyme-immunoassay(EIA)system after separation and culture of monocyte, and PGF2 was examined by PGE2 EIA system after culture of gingival fibroblasts.
The results were as follows:
1. In the MTT test of gingival fibroblasts. the change of optical density Ryas decreased significantly at 2 % of hutylalcohol extracts and 0.04 %, 0.1 %, 0.2 %,0.4 %,1 %. 2 % of ethvlacetate extracts, (p (0.05)
2. In the MTT test of periodontal ligament cells, the change of optical density were not differ significantly, but hutylalcohol and ethylacetate extracts except from butylalcohol 0.01 % showed high cell cytotoxity.
3. Both ethylacetate and butylalcohol extracts from Pulsatilla koreana inhibited the synthesis of IL-1β /3 and inhibition effect of ethylacetate extracts were higher than hutylalcohol extracts.
4. Both ethylacetate and hutylalcohol extracts from Pulsatilla koreana inhibited the synthesis of PGE2. and ethvlacetate extracts were higher than hutylalcohol extracts.
In conclusion, ethylacetate and hutylalcohol extracts from Pulsatilla koreana showed little cell cytotoxity for gingival and periodontal ligament fibroblasts, and the inhibition of IL-1(3 and PGE2 synthesis. therefore it is considered that these extracts can be developed as the therapeutics of the periodontal disease.
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